Table 1
Characteristics of the primer/probe assays used for the detection of Solea solea, Pleuronectes platessa, Misgurnus fossilis, Misgurnus bipartitus, Xenopus laevis, and Lithobates catesbeianus, respectively.
| Species | Gene | Amplicon size | Temp | Length (bp) |
|---|---|---|---|---|
| Solea solea | COI | 100 bp | 56 ∘C | |
| Forward: Ss COI F | 5′-CCCCTGCTTTCCTGCTACTT-3′ | 20 | ||
| Reverse: Ss COI R | 5′-GCATGGGCGAGGTTACTTGA-3′ | 20 | ||
| Probe: Ss COI P | 5′-ACCTCATCCGTTGTTGAAGC-3′ | 20 | ||
| Maes et al. (2024) | ||||
| Pleuronectes platessa | CytB | 90 bp | 56 ∘C | |
| Forward: Plepla CytB F | 5′-TAGGCTTCGCAGTCCTCCTC-3′ | 20 | ||
| Reverse: Plepla CytB R | 5′-TTGCAGGCGTGAAGTTGTCT-3′ | 22 | ||
| Probe: Plepla CytB P | 5′-CTAAAAGATTTGGGGAAAATAGGGCGAGT-3′ | 29 | ||
| Knudsen et al. (2019), Maes et al. (2024) | ||||
| Misgurnus fossilis | COI | 119 bp | 56 ∘C | |
| Forward: Mf COI F | 5′-CCCCCGACATAGCATTTCCG-3′ | 20 | ||
| Reverse: Mf COI R | 5′-AACTGTTCAGCCTGTCCCAG-3′ | 20 | ||
| Probe: Mf COI P | 5′-CTCGTTCCTCCTTCTGCTGG-3′ | 20 | ||
| Brys et al. (2021) | ||||
| Misgurnus bipartitus | COI | 154 bp | 56 ∘C | |
| Forward: Mb COI F | 5′-TTTAGCCGGGGTTTCGTCT-3′ | 19 | ||
| Reverse: Mb COI R | 5′-TAGGACCGGCAAGGAGAGT-3′ | 19 | ||
| Probe: Mb COI P | 5′-ACCAGACCCCCTTGTTTGT-3′ | 19 | ||
| Brys et al., (2021) | ||||
| Xenopus laevis | 12S | 83 bp | 53 ∘C | |
| Forward: SPYGEN XenLea F | 5′-AGGCTTAATGATTTTGCATC-3′ | 20 | ||
| Reverse: SPYGEN XenLea R | 5′-AGGGTATAGAAAATGTAGCC-3′ | 20 | ||
| Probe: SPYGEN XenLea P | 5′-ACGTCAGGTCAAGGTGTAGCA-3′ | 21 | ||
| Secondi et al. (2016) | ||||
| Lithobates catesbeianus | 16S | 120 bp | 55 ∘C | |
| Forward: qLC16S F | 5′-GCAGAGATAACCTCTCGT-3′ | 18 | ||
| Reverse: qLC16S R | 5′-GTCCCATAGGACTGTTCT-3′ | 18 | ||
| Probe: qLC16S P | 5′-TGCCCTCCCGAAACTAAGTGAGC-3′ | 23 | ||
| Lin et al. (2019) | ||||
| Lithobates catesbeianus | CytB | 84 bp | 60 ∘C | |
| Forward: Bullfrog F | 5′-TTTTCACTTCATCCTCCCGTTT-3′ | 22 | ||
| Reverse: Bullfrog R | 5′-GGGTTGGATGAGCCAGTTTG-3′ | 20 | ||
| Probe: Bullfrog BHQ P | 5′-TTATCGCAGCAGCAAGTATGATCCACC-3′ | 27 | ||
| Everts et al. (2021), Strickler et al. (2015), Brys et al. (2023) | ||||
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