Open Access
Issue
Knowl. Manag. Aquat. Ecosyst.
Number 422, 2021
Article Number 36
Number of page(s) 7
DOI https://doi.org/10.1051/kmae/2021035
Published online 16 November 2021

© V.E. Prie and X. Cucherat, Published by EDP Sciences 2021

Licence Creative CommonsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License CC-BY-ND (https://creativecommons.org/licenses/by-nd/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. If you remix, transform, or build upon the material, you may not distribute the modified material.

The springsnail Bythinella viridis (Poiret, 1801) is endemic to France and protected by French law. According to available data, this species is restricted to a few springs in the Oise and Marne River catchments (Falkner et al., 2002). Bythinella carinulata (Drouët, 1867), also protected by French law was described from a large area around the city of Dijon, including the Aube and Haute-Marne departments, i.e., south of the distribution area of B. viridis. B. lancelevei Locard, 1884 was described from specimens collected in the lower Seine, a few tens of kilometers away from the sea, about 200 km west of the distribution area of B. viridis.

Bythinella carinulata (Drouët, 1867) is revalidated by Boeters (1998) from material collected in the upper Seine drainage. B. lancelevei is morphologically very close, if not identical, to B. viridis, but Falkner et al. (2002) consider it as a distinct species, based on the fact that it occurs 200 km away from the nearest localities of B. viridis. For the establishment of the IUCN red list, Bichain and Prié (2010) considered B. lancelevei as a synonym of B. viridis based on the molecular results of Bichain (2007a,b). Benke et al. (2009) published a review of the phylogeography of Bythinella species throughout Europe based on molecular data and their results suggest B. viridis and B. lancelevei should be synonymized, but the authors do not discuss the taxonomic implications of their results. In the absence of a taxonomic conclusion by Benke et al. (2009), Gargominy et al. (2011) maintained B. lancelevei and B. carinulata as distinct species in the French checklist. More recently, Pouchard and Bichain (2013) and Pouchard (2018) have published new localities for B. lancelevei. Finally, the two electronic taxonomic registers TAXREF (French register, Gargominy et al., 2020) and MolluscaBase (global register of molluscs, MolluscaBase 2021) follow the validity of that species.

In this note we give additional molecular data from 15 localities from north-east France and address the question of the taxonomic status of the currently valid species in the Bythinella viridis group.

Family Bythinellidae Locard, 1893

Genus Bythinella Moquin-Tandon, 1856

Bythinella viridis (Poiret, 1801)

Orignal combination: Bulimus viridis Poiret, 1801: 45–46.

Type locality: « Le ruisseau qui tombe en cascade de la montagne en bas de laquelle est situé le moulin de Veau, proche de Chartreuve [nowadays Chery-Chartreuve, in the Aisne department] »

Distribution: according to Boeters (1998) and Falkner et al. (2002), restricted to a few springs in the Oise and Marne Rivers catchments. The type locality of B. viridis is unambiguous and still hosts the species (locality 1 in Tab. 1). COI sequences from topotypes are provided by Benke et al. (2009) and Bichain et al. (2007b); ITS sequence fragments come from Bichain et al. (2007a). Bythinella viridis is protected by French law and categorized as ENB1ab(iii,iv)+2ab(iii,iv) by the IUCN red list (Bichain and Prié, 2010).

Bythinella lancelevei Locard, 1884

Orignal combination: Bythinella lancelevei Locard, 1884.

Type locality: Villequier (Seine-Maritime), « (…)sous les pierres submergées du ruisseau du chemin du Nord, qui coule vers la Seine ».

Distribution: according to Falkner et al. (2002), known only from the type locality, but Pouchard and Bichain (2013) and Pouchard (2018) give a few more locations in the Eure and Seine-Maritime departments. Specimens of B. lancelevei collected from the spring of La Roquette in Villequier are here considered as topotypes. COI, 16S and 28S genes fragments were amplified by Benke et al. (2009). Additional COI gene fragments from three specimens sampled in Fort-Movil, about 30 km south-west of the type locality, were included in the analysis (Fig. 1, Tab. 1).

Bythinella lancelevei was considered as a synonym of B. viridis by Germain (1931), but re-established by Falkner et al. (2002). B. lancelevei is considered synonym of B. viridis by the IUCN red list (Bichain and Prié, 2010).

Bythinella carinulata (Drouët, 1867)

Orignal combination: Hydrobia carinulata Drouët, 1867: 90–91.

Type locality: « Habite les sources au pied de la Côte (calcaire jurassique), sur les pierres: fontaine de Larrey, près Dijon (type) ! source de la Norges ! la Douix, à Chatillon-sur-Seine !... (…) Je l'ai recueilli dans l'Aube et la Haute Marne dans les mêmes circonstances ».

B. carinulata has five recognized subjective synonyms.

  • Turbo griseus Vallot, 1801. Vallot (1801) does not give any precise type locality, but the original publication deals with the Côte d'Or department). The name Turbo griseus was considered nomen oblitum by Boeters (1974) and Falkner et al. (2002) considered that the strict application of the priority was not advisable in this case.

  • Paludinella turgidula Paladilhe, 1869. The type locality is Billy-les-Chanceaux (source de la Seine) and Paladilhe (1869) adds « dans le département de l'Aube, aux environs de Bar-sur-Seine et des Riceys ».

  • Paludinella scalarina Paladilhe, 1876. Châtillon-sur-Seine (Côte d'Or) and Boutigny (Seine-et-Marne).

  • Bythinella burgundina Locard, 1893. Locard (1893) attributes this name to Beaudouin (1890) [sic], probably referring to Beaudouin (1888). This nominal species is known from pits in Châtillon-sur-Seine (Côte d'Or).

  • Bythinella viridiformis R. Bernasconi, 1989. The type locality is Barbirey-sur-Ouche (Côte-d'Or). Bernasconi (1989) gives a large distribution including the Plateau de Langres and the departements of the Côte-d'Or, Haute-Marne, Haute-Saône and Vosges.

Boeters (1998) re-established the name Bythinella carinulata and considered Paludinella turgidula Paladilhe, 1869, from Ricey and Bar-sur-Seine (Aube department) as a junior synonym. According to Falkner et al. (2002), the specimens from Barbirey-sur-Ouche (Côte-d'Or department) studied by Bernasconi (1989) would also belong to B. carinulata, although Bernasconi himself eventually considered them as synonyms of B. viridis (Bernasconi com. pers. in Falkner et al., 2002). B. carinulata viridiformis is considered by Fauna Europaea as synonym of B. carinulata, but is not considered in MolluscaBase.

Distribution: Drouët (1867) gives a wide range for this species, encompassing the drainages of the Seine and the Rhône Rivers (Fig. 1).

The type locality of Bythinella carinulata (“fontaine de Larrey, près Dijon”) should be the actual Fontaine d'Ouches, which springs north of the Larrey district, in the city of Dijon. No Bythinella live in this spring anymore (E. Farat, com. pers.). According to Boeters (1998), the populations from Bar-sur-Seine are conspecific with B. carinulata. Benke et al. (2009) used specimens collected in July-sur-Sarce, which lies about five kilometers away from Bar-sur-Seine, and considered them as B. carinulata. This later locality can be used as ergonymotope (sensus Frétey et al., 2018) for this species. We used the COI, 16S and 28S genes fragments amplified by Benke et al. (2009) for B. carinulata. Bythinella carinulata is protected by French law and categorized as EN B1ab(iii)+2ab(iii) by the IUCN red list (Prié, 2010).

1 Material and methods

Morphological characters and anatomy are not relevant for species delimitation in the Bythinella genus (Bichain et al., 2007a, b). We thus relied on the genetic distances between specimens to delimit species. Following a barcoding approach, we used the pairwise distribution of the genetic distances calculated with the Barcode gene COI to identify a gap in the distribution, referred to as the “barcoding gap” (Puillandre et al., 2012), between intra and interspecific distances. A total of 1074 COI sequences of Bythinella species downloaded from GenBank (Dataset 1) were used to estimate the barcoding gap (supplementary material 1). MEGA 10.05 (Tamura et al., 2011) was used to calculate the p-distances (following Srivathsana and Meier, 2012) between each pair of specimens. The distribution of the genetic distances was then calculated using an excel sheet (Supplementary material 1). The COI barcoding gap for the Bythinella genus was found to be around 3%. This result is congruent with the species level divergence observed in other Bythinella taxonomic papers (Bichain et al., 2007a, b; Prié and Bichain, 2009).

We analyzed a total of 48 specimens from 18 localities (Tab. 1). Extraction and amplification were performed using standard protocols (ex. Bichain et al., 2007b). Total genomic DNA was extracted using the Qiagen DNA Mini Kit and following the manufacturer's protocol. Universal primers were used for the amplification of 16S (Palumbi et al., 1991) and 28S (Park and O'Foighil, 2000). For ITS and COI, we used the primers designed by Bichain et al. (2007a, b).

Sequences were automatically aligned using CLUSTALW multiple alignments implemented in BIOEDIT 7.2.5 (Hall, 1999). The accuracy of automatic alignments was confirmed by eye. Only a few gaps, unambiguously aligned, were inferred for the 28S, ITS and 16S genes: they were conserved for the analyses.

We obtained 34 sequences for the COI gene (Tab. 1), resulting after alignment in a 557 bp fragment. Seven additional sequences downloaded from GenBank were included the dataset (two sequences for both B. viridis and B. lancelevei, three sequences for B. carinulata). A total of 25 sequences were obtained for the 16S gene, and four additional sequences were included in the dataset (two for B. viridis, one for B. carinulata and one for B. lancelevei), resulting after alignment in a 502 bp fragment. Three sequences were obtained for the 28S gene, and two additional sequences are available from GenBank (one for B. lancelevei and one for B. carinulata). After alignment, 28S gene sequences resulted in a 799 bp fragment. Fourteen sequences were obtained for the ITS gene, and two additional sequences (B. viridis) were downloaded from GenBank. After alignment, ITS gene sequences resulted in a 267 bp fragment.

For phylogenetic analyses of the COI gene fragment, B. badensis Boeters, 1981 and B. cebennensis (Dupuy, 1849) were chosen as outgroups, following the results of Benke et al. (2009). Bayesian analysis were performed running two parallel analyses in MRBAYES V 3.1.2 (Huelsenbeck et al., 2001), each consisting of two Markov chains of 5 000 000 generations each, sampled every 1 000 generations. We defined three partitions (one for each codon position). Convergence of the analysis was checked using TRACER V 1.7.1 (Rambaut et al., 2018); all ESS values were> 200 (default burnin). A consensus tree was then calculated after omitting the first 10% trees as burn-in. Chains had converged after 3.000.000 generations.

2 Results

COI gene phylogeny of the genus Bythinella (based on Dataset 1) confirmed the monophyly of all studied populations (results not shown). Within this clade, corresponding to clade V of Benke et al. (2009), B. viridis and B. lancelevei topotypes could not be distinguished. Some populations of the putative B. carinulata formed distinct supported clade, but these were not monophyletic (Fig. 2). These results confirm those of Benke et al. (2009). The 16S gene was little variable (overall p-distance = 0.01). The topotypes of B. viridis and B. lancelevei shared the same haplotype for this gene. Regarding the nuclear genes, all the specimen studied shared the same haplotype for the 28S fragment. The ITS gene fragment was a bit more variable (overall p-distance = 0.07). Topotype specimens of B. viridis had an insertion (AGA) in position 99 and a G in position 238. For all gene fragments, nominal species and newly sequenced specimens could not be distinguished.

Average genetic distance for COI gene was 0.0093. Maximum distance observed was 0. 0250 between specimens of B. carinulata from July-sur-Sarce (ergonymotope, locality 14) and specimens presumably of the same species from Vals-des-Tilles (locality 17, the closest locality to the type locality in Dijon) The genetic distance between B. viridis, B. lancelevei and B. carinulata was below the barcoding gap threshold, suggesting that all the specimens analyzed belong to the same species.

thumbnail Fig. 2

Phylogenetic tree based on Bayesian inference, using the COI gene. Only posterior probabilities > 0.8 are shown. Numbers refer to the sampled localities (Fig. 1, Tab. 1). Scale bar (0.3) indicate the minimum inter-specific genetic divergence for the COI gene (barcoding gap). Top left: distribution of the genetic divergence within the genus Bythinella illustrating the barcoding gap.

3 Discussion

B. viridis is the oldest name available. In our opinion, it makes no doubt that B. lancelevei is a junior synonym of B. viridis. There is no morphological argument to jeopardize the genetic data. B. lancelevei was considered a distinct species by Falkner et al. (2002) only based on one argument: the geographic distance from B. viridis. However, (i) the known populations of B. lancelevei are in the same drainage as the known populations of B. viridis (Seine River drainage) and (ii) some localities may have been overlooked in between. The apparent isolation between the populations of the lower Seine (B. lancelevei) and the populations from the upper Seine drainage (B. viridis) could result from human impact or other recent geographic isolation, but is very likely a collection bias. Regarding B. carinulata, although all the specimens studied formed a distinct clade in COI and 16S, genetic divergence was very low, suggesting it should also be synonymized with B. viridis.

In the absence of contradictory evidence, we propose to synonymize B. viridis, B. lancelevei and B. carinulata under the name Bythinella viridis (Poiret, 1891). As B. viridis is protected in France, conservation measures and consideration in impact studies should apply throughout its the entire range, i.e., from the lower Seine west (Fig. 1, 13) to the Meuse drainage east (Fig. 1, 11) and down to the upper Rhône south (Fig. 1, 17).

Acknowledgments

This study was performed with financial support of the DREAL Picardie, and the Parc naturel régional de Lorraine. Sequencing of the specimens was performed by Lise-Marie Pigneur (Université de Lièges), Stephane Dreano (Université de Rennes I) and Sebastian Berg (Animal Ecology and Systematics, Justus Liebig University Giessen).

References

  • Beaudouin J. 1888. Faune malacologique vivante de l'arrondissement de Châtillon-sur-Seine (Côte-d'Or). Bull Soc Malac France 5: 377–423. [Google Scholar]
  • Benke M, Braendle M, Albrecht C, Wilke T. 2009. Pleistocene phylogeography and phylogenetic concordance in cold-adapted spring snails (Bythinella spp.). Mol Ecol 18: 890–903. [Google Scholar]
  • Bernasconi R. 1989. Die Bythinella der Süd-und West-Schweiz und von Ost-Frankreich (Gastropoda: Prosobranchia: Hydrobioidea). Folia malacologia 3: 33–54. [Google Scholar]
  • Bichain J-M., Boisselier-Dubayle M-C., Bouchet P, Samadi S. 2007a. Delimiting species in the genus Bythinella (Mollusca: Caenogastropoda: Rissooidea): a first attempt using molecular and morphometrics tools. Malacologia 49: 291–311. [Google Scholar]
  • Bichain J-M, Gaubert P, Samadi S, Boisselier-Dubayle M-C. 2007b. A gleam in the dark: Phylogenetic species delimitation in the confusing spring-snail genus Bythinella Moquin-Tandon, 1856 (Gastropoda: Rissooidea: Amnicolidae). Mol Phyl Evol 45: 927–941. [Google Scholar]
  • Bichain J-M, Prié V. 2010. Bythinella viridis. The IUCN Red List of Threatened Species. Version 2014. 3. <www.iucnredlist.org>. Downloaded on July 2021. [Google Scholar]
  • Boeters HD. 1998. Mollusca: Gastropoda: Superfamilie Rissooidea. In Schwoerbel J, Zwick P, eds. Süßwasserfauna von Mitteleuropa (Begründet von A. Brauer), G Fischer, Stuttgart, 76p. [Google Scholar]
  • Boeters HD. 1974. Die Gattung Bythinella und die Gattung Marstoniopsis in Westeuropa, I Westeuropaïsche Hydrobiidae, 4 (Prosobranchia). Malacologia 14: 271–285. [Google Scholar]
  • Drouët H. 1867. Mollusques terrestres et fluviatiles de la Côte-d'Or, Say, Paris, 126 p. [Google Scholar]
  • Falkner G, Ripken TEJ, Falkner M. 2002. Mollusques continentaux de la France. Liste de référence annotée et bibliographie. Patrimoines Naturels 52: 1–350. [Google Scholar]
  • Frétey T, Dewynter M, Ohler A-M. 2018. Onymotopes in zoological nomenclature: some additional terms, with fixation of a lectonymotope for Xenopus petersii Bocage, 1895 (Amphibia, Anura). Bionomina 13: 37–50. [Google Scholar]
  • Gargominy O, Prié V, Bichain J-M, Cucherat X, Fontaine B. 2011. Liste de référence annotée des mollusques continentaux de France. MalaCo 7: 307–382. [Google Scholar]
  • Hall TA. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41: 95–98. [Google Scholar]
  • Huelsenbeck JP, Ronquist F, Hall B. 2001. MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics 17: 754–755. [Google Scholar]
  • Locard A. 1893. Les Coquilles des eaux douces et saumâtres de France. Description des familles, genres et espèces, Paris, 327p. [Google Scholar]
  • Paladilhe A. 1869. Descriptions de quelques Paludinidées, Assiminidées et Mélanidées nouvelles. Rev Mag Zool 21: 273–284. [Google Scholar]
  • Palumbi S, Martin A, Romano S, McMillan WO, Stice L, Grabowski G. 1991. The Simple Fool's Guide to PCR, University of Hawaii, Honolulu, 45p. [Google Scholar]
  • Park J-K, O'Foighil D. 2000. Sphaeriid and Corbiculid clams represent separate heterodont bivalve radiations into freshwater environments. Mol Phyl and Evol 14: 75–88. [Google Scholar]
  • Pouchard C, Bichain J-M. 2013. Nouvelles localités pour Bythinella lancelevei Locard, 1884 (Gastropoda, Rissoidea, Bythinellidae) en Haute-Normandie (Eure, Seine-Maritime) et proposition de catégorisation UICN. MalaCo 9: 485–497. [Google Scholar]
  • Pouchard C. 2018. Contribution à la connaissance de Bythinella lancelevei Locard, 1884 (Gastropoda, Littorinimorpha, Bythinellidae): une présence avérée en Pays d'Auge établit une distribution plus occidentale et plus méridionale de l'espèce. MalaCo 14: 17–20. [Google Scholar]
  • Prié V, Bichain J-M. 2009. Phylogenetic relationships and description of a new stygobite species of Bythinella (Mollusca, Gastropoda, Caenogastropoda, Amnicolidae) from southern France. Zoosystema 31: 987–1000. [Google Scholar]
  • Prié V. 2010. Bythinella carinulata. The IUCN Red List of Threatened Species 2010: e.T3385 A9814892. https://dx.doi.org/10.2305/IUCN.UK.2010-4.RLTS.T3385A9814892.en. Downloaded on 23 September 2021. [Google Scholar]
  • Puillandre N, Lambert A, Brouillet S, Achaz G. 2012. ABGD, Automatic Barcode Gap Discovery for primary species delimitation. Mol Ecol 21: 1864–77. [Google Scholar]
  • Rambaut A, Drummond AJ, Xie D, Baele G, Suchard MA. 2018. Posterior summarisation in Bayesian phylogenetics using Tracer 1.7. Systematic Biology 67: 901–904. [Google Scholar]
  • Srivathsana A, Meier R. 2012. On the inappropriate use of Kimura-2-parameter (K2P) divergences in the DNA-barcoding literature. Cladistics 28: 190–194. [Google Scholar]
  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739. [Google Scholar]
  • Vallot JN. 1801. Exercice sur l'histoire naturelle. École centrale du département de la Côte-d'Or, Dijon, 8p. [Google Scholar]

Cite this article as: Prie VE, Cucherat X. 2021. Additional molecular data on the protected springsnail species Bythinella viridis (Poiret, 1801) (Gastropoda: Bythinellidae) suggest synonymy of related taxa. Knowl. Manag. Aquat. Ecosyst., 422, 36.

All Tables

Table 1

specimens and localities.

All Figures

thumbnail Fig. 1

Known Bythinella records in the area (black dots), sampled sites (red stars) and putative distribution of the three nominal species. Sampling sites numbers refer to Table 1. Circles: type localities; square: ergonymotope for B. carinulata. The green polygon refers to the distribution of B. carinulata according to Drouët (1867). Black dotted lines represent the major watershed limits (Seine, Meuse and Rhône drainages). Scale bar: 100 km.

In the text
thumbnail Fig. 2

Phylogenetic tree based on Bayesian inference, using the COI gene. Only posterior probabilities > 0.8 are shown. Numbers refer to the sampled localities (Fig. 1, Tab. 1). Scale bar (0.3) indicate the minimum inter-specific genetic divergence for the COI gene (barcoding gap). Top left: distribution of the genetic divergence within the genus Bythinella illustrating the barcoding gap.

In the text

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.