Supplementary Material

Fig. S1: Primer tested at the tissue samples extracted from 2 randomly selected individuals from the Dugesia sp. biomass (D1, D2) and from the chironomid larvae biomass (CH1, CH2). Abbreviations: SM – DNA Ladder (Promega); K – negative control.

Table S1. Homogeneity of gained sequences from tissue and eDNA samples with the most related taxa available in NCBI GenBank. Sequences (NCBI GenBank) used for primer design for Dugesia sp. and Chironomidae.

Table S2. Scheme of pooling samples from the experimental aquariums (Ae, Be, Ce, De) and negative control aquarium (Ee).

Table S3. Summary table of raw reads, reads clustered to zOTUs and number of zOTUs detected at all fish smears after various number of hours. Abbreviations: Ee-1s – 3 pooled samples of the first smear from three fish from negative control aquarium; AeBe-1s – pooled sample from experimental aquariums Ae and Be, first smear; AeBe-2s – pooled sample from experimental aquariums Ae and Be, second smear; CeDe-1s – pooled sample from experimental aquariums Ce and De, first smear; CeDe-2s – pooled sample from experimental aquariums Ce and De, second smear.

Table S4. Summary of all identified zOTUs with their percentage reads per sample after various number of hours. Homogeneity of gained zOTUs was identified via BLAST search. Abbreviations as in Table S3. For the assignment of individual zOTUs to taxonomic levels, please see Tab. S5.

Table S5. Taxonomic levels of each zOTU detected from the smears of fish skin mucus during experiment.

© B. Števove et al., Published by EDP Sciences 2023