Supplementary Material

Fig. S1: Differences in community-weighted mean (CWM) values (± standard error) of feeding strategy; motility; and life form functional trait categories in ciliate communities between control (C) and restored streams (N) for the one-month immersion period.

Fig. S2: Differences in community weighted mean (CWM) values (± standard error) of food source; feeding strategy; motility; mode of locomotion, life form functional trait categories in ciliate communities between control (C) and restored streams (N) during the two-month immersion period.

Fig. S3: Box plots showing selected enivironmental parameteres at control (C) and restored sites (N) for the one -month and two-months immersion period, Asterisk symbol (*) indicates statistically signification differences amnog estimated means (ANOSIM p<0.05), Upper and lower edges of the boxes are the first and third quartiles; thi line inside the box represents the median; individual dots are outliers, Abbreviation; COD- total chemical oxygen demand, N-NO₂ -nitries, P-PO³₄ -orthophosphates.

Fig. S4: Box plots showing organic matters and inorganic matter content; and chlorophyll σ concentrations at control (C) and restored sites (N) for the one -month and two-months immersion period. Asterisk symbol (*) indicates statistically signification differences amnog estimated means (ANOSIM p<0.05), Upper and lower edges of the boxes are the first and third quartiles; thi line inside the box represents the median; individual dots are outliers,

Fig. S5: Pairwise Spearman's rank correlation matrix of ciliate taxa abundance and environmental parameters for the one-month immersion period. Only taxa identified by Indicator species analysis (IndVal) were used for the purpose of correlation. Circle size reflects the magnitude of the correlation coefficient. Color represents the level of Spearman's correlations (blue means positive correlation and red means negative correlation). X represents statistically insignificant correlations (p>0.05). Abbrevations: AC- Aspidisca cicada, HO = Holophrya ovum, PV = Platyophrya vorax, VAC = Vorticella aquadulcis-complex; N = nitrites; COD = chemical oxygen demand; OM = organic matter; IM = inorganic matter; CHL = chlorophyll a concentration.

Fig. S6: Pairwise Spearman's rank correlation matrix of community weighted mean (CWM) values of functional trait categories and environmental parameters for the one-month immersion period. Only categories that were significantly different according to GLMMs were used for correlation. Circle size reflects the magnitude of the correlation coefficient. Color represents the level of Spearman's correlations (blue means positive correlation and red means negative correlation). X represents statistically insignificant correlations (p>0.05). Abbrevations: N = nitrites; COD = chemical oxygen demand; OM = organic matter; IM = inorganic matter; CHL = chlorophyll a concentration; FSB = food source “bacteria”; FSPPSM = food source “phagotrophic protists, small metazoans”; EPLF = ecosystem preference “lentic (freshwater)”; EPLLF = ecosystem preference “lentic and lotic (freshwater)”; EPS = ecosystem preference “soil”; HPBP = habitat preference “benthos, periphyton”; HPB = habitat preference “benthos”; HPP = habitat preference “planktonic”; MLFS = mode of locomotion “free swimming”; MLC = mode of locomotion “crawling”.

Fig. S7: Pairwise Spearman's rank correlation matrix of community weighted mean (CWM) values of functional trait categories and environmental parameters for the two-months immersion period. Only categories that were significantly different according to GLMMs were used for correlation. Circle size reflects the magnitude of the correlation coefficient. Color represents the level of Spearman's correlations (blue means positive correlation and red means negative correlation). X represents statistically insignificant correlations (p>0.05). Abbrevations: COD = chemical oxygen demand; COND= conductivity; ORTH= orthophosphates; OM = organic matter; IM = inorganic matter; CHL = chlorophyll a concentration; EPS = ecosystem preference “soil”; HPBP = habitat preference “benthos, periphyton”; HPB = habitat preference “benthos”.

© V. Gulin Beljak et al., Published by EDP Sciences 2023