Table 3
Frequency of mtDNA haplotypes, LDH genotypes, and microsatellite DNA gene diversity for three groups of Panjica hatchery broodstock and reference populations. Panjica hatchery groups: (Group 1) native stock characterized by DA mtDNA CR haplotype, 100/100 genotype and a very high proportion of DA alleles (> 0.99), (Group 2) admixed stock where discrepancies on different markers were detected, (Group 3) introduced stock characterized by AT mtDNA CR haplotype, 90/90 genotype and a very high proportion of AT alleles (> 0.99).
| Population | N | mtDNA haplotype frequency | (PCR-RFLP) mtDNA | LDH-C* genotypes | microsatellite DNA gene diversity | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Da28 | Da1c | Da1a | Da2a | ATcs1 | ATcs2 | ATcs3 | ATcs4 | DA | AT | 90/90 | 90/100 | 100/100 | Ho | He | HWE | FIS [95% CI] | A | Ar | ||
| Panjica River | 19 | 9 | 10 | 19 | 0.408 | 0.389 | 0.863 | −0.023 [−0.080, 0.028] | 3.83 | 3.70 ±0.60 | ||||||||||
| Panjica hatchery Group 1 | 70 | 36 | 22 | 12 | 70 | 0.655 | 0.614 | 0.283 | −0.060 [−0.097, −0.027] | 4.58 | 4.58 ±0.66 | |||||||||
| Panjica hatchery Group 2 | 66 | 22 | 5 | 9 | 2 | 27 | 1 | 7 | 48 | 11 | 0.701 | 0.682 | 0.000 | −0.046 [−0.138, −0.011] | 7.33 | 6.39 ±0.88 | ||||
| Panjica hatchery Group 3 | 5 | 1 | 4 | 5 | 0.800 | 0.682 | 0.998 | −0.064 [0.145, −0.031] | 4.67 | 3.25 ±0.21 | ||||||||||
| Panjcica hatchery (pooled) | 0.682 | 0.666 | 0.000 | −0.014 [−0.047, −0.034] | 8.58 | 4.58 ± 0.65 | ||||||||||||||
| Novel broodstock | 20 | 20 | 20 | 0.613 | 0.558 | 0.018 | −0.072 [−0.142, 0.019] | 3.92 | 3.80 ±0.70 | |||||||||||
| Braduljica hatchery | 8 | 2 | 2 | 4 | 8 | 0.760 | 0.712 | 0.735 | −0.002 [−0.107, 0.115] | 5.58 | 5.25 ±0.46 | |||||||||
| Danish hatchery | 10 | 8 | 2 | 10 | 0.799 | 0.755 | 0.395 | −0.006 [−0.249, 0.029] | 6.50 | 6.31 ±0.76 | ||||||||||
| Attersee Lake | 8 | 1 | 7 | 8 | 0.708 | 0.618 | 0.033 | −0.081 [−0.073, 0.066] | 4.92 | 4.92 ±0.60 | ||||||||||
| Trebišnjica River | 10 | 1 | 5 | 2 | 2 | 10 | 0.767 | 0.733 | 0.870 | 0.007 [−0.067, 0.096] | 6.67 | 6.67 ±0.83 | ||||||||
| AT reference samples (pooled) | 0.761 | 0.779 | 0.417 | 0.132 [−0.010, 0.078] | 10.67 | 10.7 ±0.83 | ||||||||||||||
| ∑ | 216 | 67 | 27 | 31 | 2 | 5 | 14 | 45 | 5 | 48 | 48 | 120 | ||||||||
Abbreviations: N – number of individuals; Ho – observed heterozygosity; He – expected heterozygosity; FIS – inbreeding coefficient of the combined microsatellite and LDH-C* with confidence intervals; HWE – p-values of the exact Fisher's test for departure from Hardy-Weinberg Equilibrium; A – average number of alleles per locus; Ar – allelic richness.
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