Issue
Knowl. Manag. Aquat. Ecosyst.
Number 425, 2024
Riparian ecology and management
Article Number 19
Number of page(s) 13
DOI https://doi.org/10.1051/kmae/2024014
Published online 16 October 2024

Supplementary material

Fig. S1. Restriction of the partial fragment of the LDH gene (428 bp) with the BseLI enzyme. Allele 100 of the DA lineage remains uncut, whereas allele 90 of the AT lineage was cut into two fragments (353 and 75 bp; some fragments of 75 bp were of very low concentration and hardly visible on the gel).

Fig. S2. Two-dimensional plot of Factorial Correspondence Analysis (FCA) for the entire sample-set based on 12 microsatellite loci.

Table S1. GenBank accession numbers of sequences detected in the study along with the reference sequences used for construction of the Median-joining network. Sequences detected in the Panjica hatchery are in bold.

Table S2. Statistical differences in genetic diversity values (Mann-Whitney U tests: P < 0.01) between Panjica River and three Panjica hatchery groups (1 – native, 2 – admixed, 3 – introduced stock). Abbreviations: Ho – observed heterozygosity; He – expected heterozygosity; A – average number of alleles per locus; Ar – allelic richness.

Table S3. Estimation of the number of genetic clusters from STRUCTURE runs using the ΔK method. Selected number of clusters is highlighted.

Table S4. List of mtDNA haplotype, LDH genotype, and proportion of Danubian alleles of microsatellite DNA of each individual from the Panjica hatchery broodstock. The last column indicates the group assigned to the individual (1 – native, 2 – admixed, 3 – introduced stock). Individuals assigned as native according to all markers (DA mtDNA haplotype, 100/100 genotype, and > 0.99 Danubian alleles of microsatellite DNA) are printed. Individuals highlighted in gray are those that were to be remain in the final DA native broodstock, after the analysis of parentage assessment (Tab. S5).

Table S5. Full-sib families belonging to Group 1 (native) Danubian Panjica hatchery individuals. Each row represents one family. Full Sibhip index – the family index the 1st column; Prob (Inc.) – inclusive probability for this family; Prob (Exc.) exclusive probability for this family; Ind. 1 – sample number of the first offspring of the family, Ind. 2 – sample number of the second offspring of the family. Rows containing Prob (Inc.) higher than 0.500 and Prob (Exc.) lower than 0.500 are highlighted gray, § marks the family member suggested to be excluded from the final DA native broodstock.

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© T. Veličković et al., Published by EDP Sciences 2024

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