Issue |
Knowl. Manag. Aquat. Ecosyst.
Number 425, 2024
Riparian ecology and management
|
|
---|---|---|
Article Number | 19 | |
Number of page(s) | 13 | |
DOI | https://doi.org/10.1051/kmae/2024014 | |
Published online | 16 October 2024 |
Research Paper
Population-genetics analysis of the brown trout broodstock in the “Panjica” hatchery (Serbia) and its conservation applications
1
Institute of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia
2
Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia
3
Department of Zoology, National Museum of the Czech Republic, Václavské náměstí 68, 115 79 Prague 1, Czech Republic
4
Department of Ecology, Faculty of Science, Charles University, Viničná 7, 12844 Prague 2, Czech Republic
5
Department of Organisms and Ecosystems Research, National Institute of Biology, Večna pot 121, 1000 Ljubljana, Slovenia
6
Institute of Zoology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11001 Belgrade, Serbia
* Corresponding author: david.stankovic@nib.si
Received:
19
January
2024
Accepted:
26
August
2024
Artificial propagation and stocking of brown trout is a standard practice in recreational fishery management. In recent decades, the importance of maintaining intraspecific diversity and protecting locally adapted lineages has been recognized for the species' long-term survival. The first step in selecting donors for stocking involves distinguishing native trout from non-native and introgressed individuals. The established method for discerning Atlantic hatchery strains from the wild populations involves genetic screening of individual diagnostic SNPs and microsatellite assignment tests. This study, using Serbia's Panjica hatchery as an example, illustrates the proper conduct of routine genetic screening for identifying suitable donors for supportive stocking. The broodstock and reference populations were screened using mtDNA control region, LDH nuclear gene, and 12 microsatellite loci to assess the origin, diversity, and inbreeding levels. The analysis revealed only moderate contamination with Atlantic trout and showed the regional origin of the Danubian genes – over 50% of the broodstock was composed of non-introgressed Danubian individuals tracing their origin to the Zapadna Morava River system. Additionally, the study highlighted a considerable discordance between LDH locus and microsatellites in identifying introgressed individuals, raising concerns about the sole reliance on LDH locus for the identification of Atlantic genetic origin in nuclear DNA.
Key words: molecular marker discordance / artificial propagation / Atlantic brown trout / Danubian brown trout / supplementation stocking
© T. Veličković et al., Published by EDP Sciences 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License CC-BY-ND (https://creativecommons.org/licenses/by-nd/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. If you remix, transform, or build upon the material, you may not distribute the modified material.
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