Table 1

Primers developed and used for the quick verification of the target invertebrates' DNA (Chironomidae, Dugesia sp.) presence in the experimental aquaria.

Primer Sequences (5′→3′) Tm Expected product length
Chiro_for GTAGAAAATGGAGCAGGAAC 45.6 °C
Chiro_rev GATCAAACAAATAGAGGTATTCGATC 51.8  °C 209 bp
Duge_for TTTGTCTTTACATATTGCTGGT 46.5  °C
Duge_rev AARCAGCAGCTAAAACAGGAAGAG 49.7  °C 170 bp

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