Box plots comparing the distribution of carbon, nitrogen, and sulphur stable isotope values from liver (dark brown) and muscle (orange) tissue from rainbow trout captured from the lake, river mouth and river of Chungará catchment. Solid horizontal lines represent the median values; boxes represent the interquartile range (IQR) and whiskers represent the variability beyond the IQR (i.e. Q1–1.5*IQR, Q3 + 1.5*IQR). T-test results reflect the significance of pairwise comparisons of each isotope values at individual level from liver and muscle tissue in each habitat (one sample t-test).