Supplementary material

Supplemental Information S1. Distribution of feces samples by three variables: “Area covered with uric acid”, “Freshness” and “Sampling substrate” (Tab. S1); Number of samples collected at each site and remaining after each step of the experimental procedure (Tab. S2); Community composition of mock samples used as positive controls (Tab. S3); The numerical parameters used for metabarcoding data curation (Tab. S4); NovaSeq metabarcoding DNA sequence read counts recovered from all fecal and control samples after each HTS data filtering step using the VTAM pipeline (Tab. S5). Food habits of the birds identified in this study (Tab. S9); Per-base sequence quality plots for the bird dataset (Fig. S1); Per-base sequence quality plots for (Fig. S2) the invertebrate dataset, and (Fig. S3) the plant dataset; Comparison between fecal samples photographed in the field and the results of their identification through metabarcoding (Fig. S4); DNA purity (A260/A230) measured in samples grouped based on the number of food items identified by metabarcoding (Fig. S5).

Supplemental Information S2. Taxa read counts retrieved after the last filtering step using the VTAM pipeline and their taxonomic assignment. (Tab. S6) bird dataset, (Tab. S7) invertebrate dataset and (Tab. S8) plant dataset.

© L. Fablet et al., Published by EDP Sciences 2024