Supplementary Material

Supplemental Information S1. Locus and primer information (Tab. S1-1), PCR protocols (Tab. S1-2) and cycling parameters (Tab. S1-3) of six multiplex PCRs for microsatellite analyses. PCR protocols (Tab. S1-4) and cycling parameters (Tab. S1-5) for the sequencing of microsatellite locus Cgo1016. PCR protocols (Tab. S1-6) and cycling parameters (Tab. S1-7) for mtDNA control region amplification.

Supplemental Information S2. Null allele estimates (Tab. S2-1), Hardy-Weinberg equilibrium tests (Tab S2-2) and pairwise Jost's D distances based on microsatellite (Tab. S2-3) and mtDNA sequencing (Tab S2-4) data.

Supplemental Information S3. Cottus spp. and Salmo trutta haplotypes (Tab. S3-1), haplotype diversity h (Tab. S3-2), and network of Salmo trutta haplotypes (Fig. S3-1).

Supplemental Information S4. Discriminant Analysis of Principal Components membership probabilities to genetic clusters (Fig. S4-1, Fig. S4-2) and alignment of sequenced microsatellite locus Cgo1016 (Fig. S4-3).

© T. Schmidt et al., Published by EDP Sciences 2023