Table 2
Detection limit of A. anguilla (LOD) of droplet digital PCR (ddPCR) using 6 serial dilution for three different conditions a) Singleplex ddPCR (eel's primers and eel's template) (ii) Singleplex − Duplex ddPCR (eel's primers and both A. anguilla and S. glanis templates) (iii) Duplex ddPCR (eel's and catfish's primers and both A. anguilla and S. glanis templates). The LOD is indicated in bold for each condition. Mean copies/μl (n = 3) and CV% between the three replicates are given for each dilution and each condition.
Eel DNA | Singleplex | Singleplex − Duplex | Duplex | ||||||
---|---|---|---|---|---|---|---|---|---|
Concentration (ng/μL) | Mean copies/μL | Positive replicate | CV% | Mean copies/μL | Positive replicate | CV% | Mean copies/μL | Positive replicate | CV% |
1.5 | 3219 | 100% | 5.01 | 2899 | 100% | 3.52 | 2837 | 100% | 3.06 |
0.15 | 441 | 100% | 8.67 | 253 | 100% | 2.53 | 256 | 100% | 6.78 |
0.015 | 33.13 | 100% | 4.10 | 15.96 | 100% | 1.91 | 15.46 | 100% | 13.21 |
0.0015 | 3.43 | 100% | 8.90 | 0.07 | 100% | 49.5 | 0.22 | 100% | 44.41 |
0.00015 | 0.27 | 100% | 11.2 | 0 | 0% | – | 0.02 | 33% | – |
0.000015 | 0 | 0% | – | 0 | 0% | – | 0 | 0% | – |
0.0000015 | 0 | 0% | – | 0 | 0% | – | 0 | 0% | – |
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