Table 1
Specific ddPCR primers (F and R) and probe (P) used for Anguilla anguilla and Silurus glanis detection. Probes are 5′-end modified with a fluorescent dye, and equipped with a quencher-modification at the 3′-end. Names, sequences (5'-> 3') and target fragment length (bp) are indicated.
Target species | Primer and probe name | Sequence (5' −> 3') | Lenght (bp) |
---|---|---|---|
Anguilla anguilla | Angang_F10571 | ATCTAGCAACGGACCCCTTA | 106 |
Angang_R10676b | TTGGTTGGTTCTAGCCGCA | ||
Angang_P10595 | FAM-ACACCACTACTAGTTTTATCTTGCT-BHQ1 | ||
Silurus glanis | 284F | GACTTCTCCCTCCTTCATTCCTG | 117 |
400R | AAGCACCTGCGTGGGCG | ||
Pr324F | HEX-CGGAGTCGAAGCGGGC-BHQ2 |
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