Table 1

Specific ddPCR primers (F and R) and probe (P) used for Anguilla anguilla and Silurus glanis detection. Probes are 5′-end modified with a fluorescent dye, and equipped with a quencher-modification at the 3′-end. Names, sequences (5'-> 3') and target fragment length (bp) are indicated.

Target species Primer and probe name Sequence (5' −> 3') Lenght (bp)
Anguilla anguilla Angang_F10571 ATCTAGCAACGGACCCCTTA 106
Angang_R10676b TTGGTTGGTTCTAGCCGCA
Angang_P10595 FAM-ACACCACTACTAGTTTTATCTTGCT-BHQ1
Silurus glanis 284F GACTTCTCCCTCCTTCATTCCTG 117
400R AAGCACCTGCGTGGGCG
Pr324F HEX-CGGAGTCGAAGCGGGC-BHQ2

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