Supplementary Material

Figure S1. Workflow summary of eDNA sample processing and analytical steps. Schematic representation of the workflow from field sampling to the final analytical dataset. Three field replicates were collected at each station and processed independently through extraction, amplification, sequencing, and bioinformatics. Replicates were pooled at the statistical stage to represent one composite sample per station.

Figure S2. Rarefaction curves of the crustacean community. Each line corresponds to a sampling station.

Figure S3. Sample-size-based and coverage-based rarefaction (solid line segment) and extrapolation (dotted line segments) sampling curves with 95% confidence intervals for the eDNA dataset of Réunion island by diversity order: q = 0 (species richness).

Table S1. Summary of OTU assignments across major taxonomic groups (prior to cleaning and filtering steps), showing the number and percentage of OTUs retained for Decapoda versus non-target sequences.

© L.-A. Jannel et al., Published by EDP Sciences 2026