Table 3

Genetic polymorphism measures based on the cyt b gene for Barbus balcanicus in Croatia.

Population/River catchment/Region N h Hd π η S k
Željeznica 2 2 1 0.00351 4 4 4
Žarovnica 6 3 0.733 0.00011 3 3 1.267
Bednja 8 4 0.821 0.00166 5 5 1.893
Lonjica 6 3 0.6 0.00105 3 3 1.2
Kašina 8 5 0.857 0.00128 4 4 1.464
Burnjak 6 3 0.733 0.00082 2 2 0.933
Lonja 20 8 0.884 0.00135 8 8 1.532
Sarni 6 3 0.6 0.00088 3 3 1
Rijeka 8 3 0.714 0.00088 2 2 1
Krapina 15 5 0.781 0.00117 4 4 1.333
Sava* 6 2 0.333 0.00029 1 1 0.333
Sutla 3 1 0 0 0 0 0
NORTH REGION 52 10 0.883 0.00140 10 10 1.600

Toplica 2 2 1 0.00088 1 1 1
Strančevac 3 2 0.667 0.00058 1 1 0.667
Sivornica 2 1 0 0 0 0 0
Brusnica 2 2 1 0.00176 2 2 2
Pakra 11 5 0.764 0.00102 5 5 1.164
Rinovica 2 1 0 0 0 0 0
Londža 3 2 0.667 0.00117 2 2 1.333
Kutjevačka rijeka 4 1 0 0 0 0 0
Orljava 10 4 0.733 0.00098 3 3 1.111
Putnjak 10 4 0.8 0.00099 3 3 1.133
Rešetarnica 10 4 0.8 0.00099 3 3 1.133
Šumečica 5 3 0.8 0.00088 2 2 1
Velika Radetina 1 & 2 5 3 0.8 0.00088 2 2 1
Karašica 10 4 0.778 0.00090 3 3 1.022
Česma 4 1 0 0 0 0 0
EAST REGION 45 11 0.804 0.00102 11 11 1.162

Sunja 5 3 0.8 0.00158 3 3 1.8
Listovački 3 1 0 0 0 0 0
Brusovača 5 2 0.4 0.00070 2 2 0.8
Bublen 4 3 0.833 0.00102 2 2 1.167
Glina 12 4 0.742 0.00116 3 3 1.318
CENTRAL REGION 18 7 0.869 0.00150 6 6 1.712

TOTAL SAMPLE 115 15 0.880 0.00130 16 16 1.480

N − sequence number, h − haplotype number, Hd − haplotype diversity, π − nucleotide diversity, η − total number of mutations, S − number of polymorphic sites, k − average number of nucleotide differences. At five localities (Pronjak, Dubočanka, Kravajčevca, Bijela rijeka and Stupnica (Una)), the sample size was too low to obtain reliable estimates (1), but these samples were included in the river catchment, region and total sample.

*Sava is representing only the Vidovec Stream not the whole Sava River drainage.

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