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Table 2

Test species sensitivity to the most widely used reference toxicants.

Test cultures Reference toxicants and LC

K2Cr2O7 CuSO4 Phenol
Cladoceran crustacean 48 h LC50 = 0.2–0.8 mg/L (response range) 48 h LC50 = 54.6 μg/L 48 h EC50 = 9.13 mg/L
Daphnia magna Straus   4 d LC50 = 34.2 μg/L  
  ERD*, 2014b Struewing et al., 2015 Lilius et al., 1995
Cladoceran crustacean Ceriodaphnia affinis Ceriodaphnia dubia  
Ceriodaphnia 24 h LC50 = 0.9–2.0 mg/L (response range) 48 h LC50 = 27.6 μg/L  
  ERD, 2007 7 d LC50 = 52.0 μg/L  
    Struewing et al., 2015  
Anostracan crustacean Thamnocephalus platyurus 24 h LC50 = 0.100 mg/L with 95% confidence limit of 0.052–0.148 mg/L    
  International Interlaboratory Comparison, Protocol Toxkit    
Benthic ostracod crustacean Heterocypris incongruens   6d LC50 = 5.79 mg/L  
    The results of a reference test with this compound should be in the range 2.21–9.37 mg/L  
    International Interlaboratory Comparison, Protocol Toxkit  
Amphipod crustacean 7 d LC50 = 137 μgCr/L with 95% CL 106–176 7 d LC50 = 90 μgCu/L with 95% CL 82–99  
Hyalella azteca (Cr was added in tap water as anion salt) (Cu was added in tap water as atomic absorption standards)  
  Borgmann et al., 2005 Borgmann et al., 2005  
Rotifer Brachionus calyciflorus   24 h LC50 = 0.026 mg/L with 95% CL 0.022–0.030 24 h LC50>150 mg/L
    Snell et al., 1991 Snell et al., 1991
Green micro-algae 72 h EbC50 (reduction of biomass) = 0.52 ± 0.21 mg/L    
Selenastrum capricornutum 72 h ErC50 (reduction of growth rate) = 0.84 ± 0.27 mg/L    
  International Interlaboratory Comparison (2006), Protocol Toxkit    
Green micro-algae 22 h EC50 (optical density) = 0.4–1.6 mg/L (response range)    
Chlorella vulgaris Beijer ERD, 2014a    
  22 h EC50 (delayed fluorescence) = 0.2–0.8 mg/L (response range)    
  ERD, 2014c    
Ciliate protozoan 24 d LC50 = 0.55–0.89 g/L 30 min EC50 (chemotoxic reaction) = 0.01–1.0 mg/L  
Paramecium caudatum ERD, 2007 ERD, 2015  
Ehrenberg      
Macrophyte Lemna paucicostata     72 h EC50 (growth of fronds) = 2.70 µM
      72 h EC50 (maximum quantum yield of PS II (Fv/Fm) = 1.91 µM
      Park et al., 2012
Bacteria     Bioluminescence inhibition
Aliivibrio fischeri     5 min EC50 = 16.8 mg/L
      15 min EC50 = 18.3 mg/L
      Hernando et al., 2006
*

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