Fig. 1

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Maximum likelihood (ML) estimates of phylogenetic relationships of Misgurnus sp., P. dabryanus and Cobitis taenia COI sequences (622 bp). Nodes are labeled with the highest bootstrap support by ML (1000 replications). In bold: Sequences of the identified and sequenced specimen from the oxbow at River Inn, southern Germany. All branches had bootstrap support above 60%. The scale bar shows the best fit model chosen based on the Bayesian information criterion approach (Hasegawa–Kishino–Yano model with discrete Gamma distribution, HKY+G+I, above), and substitutions/site (below). In brackets, the current GenBank accession numbers of the published sequences used for this analysis. Values beside curly brackets indicate nucleotide (π) haplotype (h) and diversity.

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