Issue |
Knowl. Manag. Aquat. Ecosyst.
Number 419, 2018
|
|
---|---|---|
Article Number | 10 | |
Number of page(s) | 4 | |
DOI | https://doi.org/10.1051/kmae/2018001 | |
Published online | 16 February 2018 |
Short Communication
Observations of crayfish plague infections in commercially important narrow-clawed crayfish populations in Turkey
Observations sur l'infection de la peste de l'écrevisse dans des populations d'écrevisses à pattes grêles d'importance commerciale en Turquie
1
Department of Environmental and Biological Sciences, University of Eastern Finland, Kuopio campus,
P.O. Box 1627,
70211
Kuopio, Suomi-Finland
2
Fisheries Faculty, Firat University,
23119
Elaziğ, Turkey
3
Gazanfer Bilge Vocational School, Kocaeli University,
Karamürsel,
Kocaeli, Turkey
4
Biological and Medical Laboratory Research, HAN University of Applied Sciences,
Kapittelweg 33 (B0.02),
6525 EN
Nijmegen, Netherlands
5
Fisheries Faculty, Munzur University,
Tunceli, Turkey
* Corresponding author: japo.jussila@uef.fi
We studied the presence of possible Aphanomyces astaci infections in eight Turkish narrow-clawed crayfish (Astacus leptodactylus) populations by analyzing the prevalence and genotypes of the disease agent A. astaci. The qPCR analyses revealed A. astaci infection in seven of the studied eight populations, with the agent level A2 or higher. The agent levels among the infected populations varied from A0 to A5, i.e., from negative to high level of infection, based on qPCR ranking. Based on the sequencing of the chitinase gene and the mitochondrial ribosomal rnnS and rnnL subunits, we detected both A (As) and B (PsI) haplogroups of A. astaci in our samples, with each of the studied populations being carriers of only one haplotype. The results confirm previous detections of A. astaci in Turkish narrow-clawed crayfish populations and reveal, that both A and B haplogroup A. astaci carriers exist widely in A. leptodactylus populations of Turkey.
Résumé
Nous avons étudié la présence possible d'Aphanomyces astaci dans huit populations turques d'écrevisses à pattes grêles (Astacus leptodactylus) en analysant la prévalence et les génotypes de l'agent pathogène A. astaci. Les analyses qPCR ont révélé une infection à A. astaci dans sept des huit populations étudiées, avec le niveau d'agent A2 ou plus. Les taux d'agents chez les populations infectées variaient de A0 à A5, c'est-à-dire du niveau négatif au niveau élevé d'infection, selon le classement qPCR. En se basant sur le séquençage du gène de la chitinase et des sous-unités rnnS et rnnL du ribosome mitochondrial, nous avons détecté les haplogroupes A (As) et B (PsI) d' A. astaci dans nos échantillons, chacune des populations étudiées n'étant porteuse que d'un seul haplotype. Les résultats confirment les détections précédentes d' A. astaci dans les populations d'écrevisses à pattes grêles et révèlent que les porteurs des deux haplogroupes d'A. astaci existent largement dans les populations d' A. leptodactylus de Turquie.
Key words: Astacus leptodactylus / Aphanomyces astaci / Turkey / Astacus leptodactylus / Aphanomyces astaci / Turquie
Mots clés : Astacus leptodactylus / Aphanomyces astaci / Turquie
© H. Kokko et al., Published by EDP Sciences 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution License CC-BY-ND (http://creativecommons.org/licenses/by-nd/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. If you remix, transform, or build upon the material, you may not distribute the modified material.
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