Interaction plots illustrating the behaviour of males (ex-ante) and the behavioural responses of males exposed (ex-post) to the four treatment waters: Calcium-enriched water (wCCE). water conditioned by sexually inactive females (wCIF), by control mature females (wCMF) and by treated mature females (wTMF). (A) Nstone: handling the air-stone as frequency, (B) Tstone: handling the air-stone as time, (C) AntFlic: antennal flicking, (D) CrayMov: crayfish moving. Different letters (a-b) indicate statistically significant differences (p < 0.05); same letters (a-a or b-b) indicate no statistically difference.