Fine-grain residual regional richness of fish species and macroinvertebrate genera among fine-grain ecoregions. Ecoregions with <10 sample sites were deemed to have insufficient data. Red-orange-yellow colors denote negative residual richness (below the predicted richness line for their number of samples). Blue-green colors denote positive residual richness. Positive residuals indicate greater than expected regional richness given the number of sampled sites; negative residuals indicate lower than expected regional richness given the number of sampled sites.