Appendix 1


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Phylogenetic tree of A. torrentium based on COI data obtained through Bayesian analysis performed in MrBayes 3.2.6 (Ronquist et al., 2012). The optimal model of nucleotide evolution for COI data set was HKY + I + G, selected under the Bayesian information criterion (BIC) using the jModelTest 2.1.10. (Darriba et al., 2012). The BA priors were set according to the suggested model; two separate runs with four Metropolis-coupled Monte Carlo Markov chains (MMCM) were performed for 10,000,000 generations, and trees were sampled every 1,000 generations. After checking congruence (ESS values > 200) with Tracer v1.7.1 (Rambaut et al., 2018), the first 25% of sampled trees were eliminated as burn-in, and a tree was constructed, with nodal values representing the posterior probabilities. Numbers at the nodes indicate Bayesian posterior probabilities (values > 0.91 are indicated). New Romanian sequences belonging to haplotype AT108 are indicated in red colour. Phylogroups are represented by different colour: black − central and south-eastern Europe (CSE), blue − Gorski Kotar (GK), purple − Lika and Dalmatia (LD), orange − Žumberak, Plitvice and Bjelolasica (ŽPB), pink − southern Balkans (SB), green − Banovina (BAN), red − Zeleni Vir (ZV), gray − Apuseni Mountain (APU) and turquoise blue − Kordun (KOR).

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