Open Access
Review
Table 5
Toxkit microbiotests for freshwater and freshwater sediments.
| Test | Test species | Hatching/Incubation conditions | Testing conditions | Culturing and dilution medium |
|---|---|---|---|---|
| Toxi-Screening kit | Freeze-dried luminescent bacteria Aliivibrio fischeri | – | At ambient temperature within the range 15 °C–25 °C | Control water |
| Chronic protoxkit F | Ciliate Tetrahymena thermophila | – | In darkness at 30 °C | Protoxit medium |
| Acute rotoxkit F | Rotifer Brachionus calyciflorus | At 25 °C, under continuous illumination (light source of min. 3000–4000 lux), 16–18 h | At 25 °C in darkness, for 24 h | Moderately hard synthetic water, US EPA formula |
| Short-chronic rotoxkit F | Rotifer Brachionus calyciflorus | At 25 °C, under continuous illumination (light source of min. 3000–4000 lux), 16–18 h | At 25 °C in darkness, for 24 h | Moderately hard synthetic water, US EPA formula |
| Daph toxkit F magna | Cladoceran crustacean Daphnia magna | At 20–22 °C under continuous illumination of min. 6000 lux, 72 h | At 20°C, in darkness | ISO medium, formula according to ISO 6341 |
| Thamno toxkit F | Anostracan crustacean Thamnocephalus platyurus | At 25 °C, under continuous illumination (light source of min. 3000–4000 lux), 20–22 h | At 25 °C in darkness, for 24 h | Moderately hard synthetic water, US EPA formula |
| Ostracod toxkit F | Benthic ostracod crustacean Heterocypris incongruens | At 25 °C, under continuous illumination (light source of min. 3000–4000 lux), 52 h | At 25 °C, in darkness, for 6 days | Moderately hard synthetic water, US EPA formula |
| Ceriodaphtoxkit | Cladoceran crustacean Ceriodaphnia dubia | At 25 °C, under continuous illumination of min. 6000 lux, about 80 h | At 25 °C, in darkness, for 24 h | Moderately hard synthetic water, US EPA formula |
| Rapid toxkit | Anostracan crustacean Thamnocephalus platyurus | At 25 °C, under continuous illumination (light source of min. 3000–4000 lux), for 30 h (minimum) to 45 h (maximum) | For 15 to 30 min at 25 °C in darkness | Moderately hard synthetic water, US EPA formula |
| Algal toxkit F | Green micro-algae Pseudokirchneriella subcapitata (formerly known as Selenastrum capricornutum and Raphidocelis subcapitata) | – | 23 °C + 2 °C with constant uniform illumination from cool white fluorescent lamps: 10 000 lux for sideway illumination of the holding tray or 3000–4000 lux for bottom illumination | Algal culturing medium with deionized water, according to the formula of the OECD guideline 201 and the ISO/DIS guideline 8692 |
| Spirodela duckweed toxkit | Macrophyte Spirodela polyrhiza | At 25 °C with continuous “top” illumination (at least 6000 lux at the surface of the petri dish) | At 25 °C with continuous illumination of 6000 lux (at the top of the multiwell), 3 days (72 h + 1 h) | The growth medium is the “Steinberg medium” prescribed by ISO for Lemna toxicity tests (ISO 20079) |
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