Open Access
Review
Table 4
Culture medium formulations for macrophytes and phytoplankton.
| For macrophytes | For phytoplankton (algae and cyanobacteria) | |||||
|---|---|---|---|---|---|---|
| Salts | Swedish Standard (SIS) Lemna growth medium according to OECD 221 | 20X AAP growth medium according to OECD 221 | Salts | Original medium from OECD TG 201 according to ISO 8692 | US EPA medium AAP according to ASTM | Uspensky's medium according to ERD, 2011 |
| Concentration, mg/L | Concentration, mg/L | |||||
| NaNO3 | 85 | 510 | NaHCO3 | 50 | 15.0 | – |
| Na2CO3 | 20 | – | NaNO3 | – | 25.5 | – |
| NaHCO3 | – | 300 | NH4Cl | 15 | – | – |
| KH2PO4 | 13.4 | – | MgCl2·6H2O | 12 | 12.16 | – |
| K2HPO4 · 3H2O | – | 30 | CaCl2·2H2O | 18 | 4.41 | – |
| MgSO4 · 7H2O | 75 | 290 | MgSO4·7H2O | 15 | 14.6 | 25 |
| CaCl2 · 2H2O | 36 | 90 | KH2PO4 | 1.6 | – | – |
| H3BO3 | 1.0 | 3.7 | K2HPO4 | – | 1.044 | 25 |
| MnCl2·4H2O | 0.2 | 8.3 | KNO3 | – | – | 25 |
| MnCl2·6H2O | – | 240 | K2CO3 | – | – | 34.5 |
| Na2MoO4·2H2O | 0.01 | 145 μg/L | FeCl3·6H2O | 0.064 | 0.160 | – |
| ZnSO4·7H2O | 0.05 | – | H2BO3 | 0.185 | 0.186 | 2.86 |
| ZnCl2 | – | 66 μg/L | MnCl2 | 0.415 | 0.415 | 1.81 |
| CuSO4·5H2O | 0.005 | – | ZnCl2 | 0.003 | 0.00327 | |
| CuCl2·2H2O | – | 0.24 μg/L | ZnSO4·7H2O | – | – | 0.222 |
| Co(NO3)2·6H2O | 0.01 | – | CoCl2·6H2O | 0.0015 | 0.00143 | – |
| CoCl2·6H2O | – | 29 μg/L | CuCl2·2H2O | 0.00001 | 0.000012 | – |
| FeCl3·6H2O | 0.84 | 3.2 | Na2MoO4·2H2O | 0.007 | 0.00726 | – |
| Na2-EDTA·2H2O | 1.4 | 6.0 | Na2-EDTA·2H2O | 0.100 | 0.300 | – |
| MOPS | 490 | – | MoO3 | – | – | 0.01764 |
| – | – | – | NH4VO | – | – | 0.02296 |
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