Observed and expected restriction patterns of the COI PCR products of A. pallipes samples in Spanish populations with HpaI and CviAII. Lines 1 and 7 – in each gel – and lines 1 and 6 – in each scheme – show the GeneRuler^TM 100 bp DNA Ladder (Thermo Scientific). Asterisks indicate the 500 bp and 1000 bp bands. Patterns A, B, C and D are detailed in Table 1. Ctrl: Restriction control.