Table 2

Results of ANOSIM and SIMPER analyses showing prey categories with the highest contribution (%) to between-year average dissimilarity (AvD) in the diet of small (30–60 mm SL) and large (61–90 mm SL) pumpkinseed during the establishment (1985) and integration stages (2003 and 2013) of the invasion in the mainstem of the Guadiana river (Portugal). Rank order of contribution is represented in parentheses.

AvD Copepoda Chironomidae Cladocera Hemiptera Ephemeroptera Trichoptera
Between years
Small fish (R = 0.578; P< 0.001)
1985 vs. 2003 (R = 0.665; P< 0.001) 77.40 24.14 (1) 19.86 (3) 22.03 (2)
1985 vs. 2013 (R = 0.348; P = 0.005) 61.18 21.75 (1) 18.09 (2) 16.12 (3)
2003 vs. 2013 (R = 0.843; P = 0.002) 72.86 26.44 (2) 27.31 (1)

Large fish (R = 0.779; P< 0.001)
1985 vs. 2003 (R = 0.687; P = 0.003) 66.24 25.42 (1) 25.36 (2)
1985 vs. 2013 (R = 0.995; P< 0.001) 53.60 20.57 (2) 15.83 (3) 28.62 (1)
2003 vs. 2013 (R = 0.796; P = 0.002) 68.13 25.06 (1) 14.77 (3) 20.76 (2)

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