Table 1

Summary of crustacean families and species detected by environmental DNA (eDNA) metabarcoding across sampling stations on Réunion Island. Crosses indicate detections. “C1” and “C2” refer to the two sampling campaigns (C1: July 2022 and C2: December 2024). Taxa are grouped into three ecological categories: amphidromous, marine, and terrestrial.

Amphidromous Marine Terrestrial


Atyidae Palaemonidae Varunidae Penaeidae Gecarcinidae Grapsidae



Watershed Site Atyoida serrata Atyoida cf. serrata Caridina henriettae Caridina typus Halocaridinides sp. Macrobrachium australe Macrobrachium lar Macrobrachium sp. Palaemon concinnus Varuna litterata Metapenaeus sp. Penaeus indicus Cardisoma carnifex Geograpsus grayi Species Richness
Rivière du Mât C1_MAT_1 X 1
C1_MAT_2 X 1
C1_MAT_3 X 1
C2_MAT_1 X X 2
C2_MAT_2 X 1
C2_MAT_3 X X 2
Rivière des Marsouins C1_MAR_1 X X X 3
C1_MAR_2 X 1
C2 MAR_1 X X x 3
SaintEtienne C1_STE_1 X 1
C2_STE_1 X X X X x 5
C1_GB_1 X 1
C1_PR_1 X 1
Ravine Saint-Gilles C1_GIL_1 X X X X X 5
C2_GIL_1 X X X 3
Rivière des Roches C1_ROC_1 X X X X 4
C2_ROC_1 X X X X X X 6
Rivière Saint-Denis C1_DEN_1 X X X X X 5
C2 DEN_1 X X X 3
SainteMarie C1 STM 1 X X X X 4
Langevin C1_LAN_1 X X X X 4
C2 LAN 1 X X X 3
Remparts C1_REM_1 X X X 3
C2_REM_1 X X 2
Rivière des pluies C1_PLU_1 X X X X 4
C2_PLU_1 X X 2
SainteSuzanne C1_SUZ_1 X X X 3
Saint-Jean C1 STJ 1 X X 2
Anse des Cascades C1_CAS_1 X X X X 4
Saint-Paul C1_CIM_1 X X X 3

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